Novel polymorphisms in the Alpha-Lactalbumin (Lalba) gene and analysis of gene expression in dairy buffaloes (Bubalus bubalis) of the Amazon

Novel polymorphisms in the Alpha-Lactalbumin (Lalba) gene and analysis of gene expression in dairy buffaloes (Bubalus bubalis) of the Amazon

Blog Article

This research aimed to find new SNPs in the Lalba gene to associate them with milk production and analyze their expression profile.A total of 104 females from a property located in Bujaru/PA, were used.DNA extractions were performed using the phenolic method, followed by PCR, purification, and pomyslnaszycie.com sequencing to investigate SNPs in the promoter region and 5’ UTR.RNA was extracted from milk epithelial cells to determine the expression profile of Lalba in 7 haplotypes selected for average milk production.

Ten new SNPs were found in the studied region, with emphasis on the indel (-555>TAAA) observed in 7% of the population.None of the SNPs showed a significant association with average milk production (ML).Seven SNPs showed wild-type nucleotide allele frequencies greater than 0.5, with 90% of them detected as HWE.

The SNP-42 (A>G) showed a strong binding site for the transcription factor HNF3.Haplotypes 4 and 2 were significant when compared to the others and had higher expression.Although no SNP influenced milk production, haplotypes 4 and 2 showed the opposite, as they presented higher levels of berness white sneakers mRNA expression in somatic milk cells, which is probably due to the additive effect of the combined alleles and, therefore, are desirable to be fixed in the population.However, studies focusing on the relationship between SNPs and expression levels in these cells, together with other genes that may be influencing milk production levels, should be carried out to investigate whether the phenotypes of the animals confirm these variants.